糜蛋白酶素

作用与用途

Biochem/physiol Actions Chymostatin is a strong inhibitor of many proteases, including chymotrypsin, papain, chymotrypsin-like serine proteinases, chymases, and lysosomal cysteine proteinases such as cathepsins A,B,C, B, H, and L. It weakly inhibits human leucocyte elastase. It is effective at a final concentration of 100 to 200 μg/ml (10 to 100 μM). Chymostatin is often included in protease inhibitor cocktails used with plant extracts.
Other Notes A mixture of A (major), B and C components. A: X=Leu; B: X=Val; C: X=Ile
chymostatin A MW = 607.7
chymostatin B MW = 593.7
chymostatin C MW = 607.7
Preparation Note Solubility testing in glacial acetic acid at 10 mg/ml yields a clear solution, which is usually colorless, but can be yellow in appearance. It is reportedly also soluble in DMSO; only slightly soluble in water and short-chain alcohols; insoluble in ethyl acetate, butyl acetate, ether, hexane, and petroleum ether. Stock solutions (10 mM) can be prepared in DMSO and are stable for months at -20 °C. Stock solutions can also be made in 0.1 M HCl. Dilute solutions (10-100 μM) are only stable for several hours, due to oxidation of the terminal aldehyde.

贮存方法

-20°C密闭，避光，通风干燥处

毒理学数据

毒理学数据：
1、急性毒性：
小鼠经口LD50：>500 mg/kg；
小鼠腹腔LD50：>500 mg/kg；

计算化学数据

计算化学数据：
1、 疏水参数计算参考值（XlogP）：未确定
2、 氢键供体数量：21
3、 氢键受体数量：27
4、 可旋转化学键数量：44
5、 拓扑分子极性表面积（TPSA）：612
6、 重原子数量：131
7、 表面电荷：0
8、 复杂度：3010
9、 同位素原子数量：0
10、 确定原子立构中心数量：13
11、 不确定原子立构中心数量：3
12、 确定化学键立构中心数量：0
13、 不确定化学键立构中心数量：0
14、 共价键单元数量：1

物理性质

性状：白色片状结晶。
熔点（oC）：>300
溶解性：溶于水和乙醇。