核糖核酸酶T1(米曲霉)

物理性质

性状:硫酸铵悬浮液。
溶解性：溶于水

作用与用途

Analysis Note Protein determined by E1%/280
Unit Definition One unit will produce acid soluble oligonucleotides equivalent to a ΔA260 of 1.0 in 15 min at pH 7.5 at 37°C, in a reaction volume of 1.0 mL. Substrate: Yeast RNA.
Physical form Suspension in 3.2 M (NH4)2SO4 solution, pH ~ 6
Biochem/physiol Actions Catalyzes the two-stage endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotides with 2',3'-cyclic phosphate intermediates. Differs from Pancreatic RNase in that it attacks the guanine sites specifically to yield 3′-GMP and oligonucleotides with a 3′-GMP terminal group.

贮存方法

2-8°C密闭、阴凉干燥处

毒理学数据

致突变数据：
微生物突变试验：细菌-鼠伤寒沙门氏菌当剂量达到100mg/L引起突变。

生态学数据

通常对水体是稍微有害的，不要将未稀释或大量产品接触地下水，水道或污水系统，未经政府许可勿将材料排入周围环境。