酰基转移酶I(猪肾)

物理性质

性状：浅红色或浅黄色冻干粉。
溶解性：溶于水，最适pH值7.0-7.5(猪肾、淀粉酶)或7-8(米曲酶)。

作用与用途

Analysis Note Protein determined by biuret.
Unit Definition One unit will hydrolyze 1.0 μmole of N-acetyl-L-methionine per hr at pH 7.0 at 25 °C.
Biochem/physiol Actions The enzyme hydrolyzes a variety of N-acyl-L-amino acids and dehydropeptides containing dehydroalanyl residues. N-acyl-D-amino acids and dehydropeptides other than dehydroalanine are not hydrolyzed. The following compounds are hydrolyzed by acylase I: chloroacetyl-L-amino acids, L-leucinamide, chloroacetyldehydroalanine, glycyl-L-amino acids, and glycyldehydroalanine. Aminoacylase is widely used as a reagent to resolve amino acid racemates. The Km for acyl amino acids have been reported in the literature: Ac-L-Met (0.99 mM), Ac-L-Glu (10.2 mM), and Ac-L-Phe (5.5 mM).
Aminoacylase from pig kidney is activated by Zn2+ and Co2+ and inhibited by SH blocking reagents, disulfide reducing reagents, N-tosyl-L-alanine,
DL-norleucine, and N-tosyl-L-lysine chloromethylketone.

性质与稳定性

用以分离D-型和L-型氨基酸。

合成方法

以猪肾、淀粉酶或米曲霉为原料，经匀浆、离心去沉淀后，用硫酸铵分级沉淀，经水透析、离心后得粗酶液，再分别用丙酮、乙醇分级沉淀、DEAE-纤维素柱层析纯化后得产品。

贮存方法

密封-20℃干燥保存。